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【文獻(xiàn)標(biāo)題】Anti-Inflammatory Activity of Water-Soluble Polysaccharide of Agaricus blazei Murill on Ovariectomized Osteopenic Rats

【作者】Peng Wang, Xiao-Tao Li, Lei Sun et.al

【作者單位】 Harbin Medical University（哈爾濱醫(yī)科大學(xué)）

【文獻(xiàn)中引用產(chǎn)品】

大鼠白介素1β (IL-1β)ELISA試劑盒

大鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【關(guān)鍵詞】無

【DOI】doi.org/10.1155/2013/164817

【出版期刊】《Evidence-Based Complementary and Alternative Medicine》

【產(chǎn)品原文引用】

Estimation of Serum IL-1β and TNF-α Level. Serum samples were disintegrated in 5 volumes of ice-cold RIPA buffer. After incubation on ice for 30 minutes, samples were centrifuged twice at 20,000 Xg for 15 minutes at 4°C. The resulting supernatants were used for assay. The concentration of IL-1β and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.

【文獻(xiàn)標(biāo)題】Tissuefactorpathwayinhibito-rgenetransferprevents vascularsmoothmusclecellproliferationbyinterfering withtheMCP-3/CCR2pathway

【作者】YuFu,DandanMa et.al

【作者單位】哈爾濱醫(yī)科大學(xué)第一附屬醫(yī)院（The First Affiliated Hospital of Harbin Medical University）

【文獻(xiàn)中引用產(chǎn)品】

小鼠溴脫氧核苷尿嘧-啶(BrdU)ELISA試劑盒

【DOI】doi.org/10.1038/labinvest.2015.106

【影響因子(IF)】5.51

【出版期刊】《LaboratoryInvestigation》

【產(chǎn)品原文引用】VSMC proliferation was analyzed via measuring DNA

synthesis with a colorimetric bromodeoxyuridine (BrdU)

ELISA kit according to the manufacturer’s instructions

(Shanghai JinMa Laboratory and Equipment Company,

Shanghai, China).

【文獻(xiàn)標(biāo)題】Tissue factor pathway inhibitor gene transfer prevents vascular smooth muscle cell proliferation by interfering with the MCP-3/CCR2 pathway

【作者】Yu Fu, Dandan Ma, Yue Liu,et.al

【作者單位】哈爾濱醫(yī)科大學(xué)( Harbin Medical University)

【文獻(xiàn)中引用產(chǎn)品】

小鼠溴脫氧核苷尿嘧-啶(BrdU)ELISA試劑盒

小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【DOI】doi:10.1038/labinvest.2015.106

【影響因子(IF)】6.7

【出版期刊】《Laboratory Investigation》

【產(chǎn)品原文引用】

Cell Proliferation StudiesVSMC proliferation was analyzed via measuring DNA synthesis with a colorimetric bromodeoxyuridine (BrdU) ELISA kit according to the manufacturer’s instructions (Shanghai JinMa Laboratory and Equipment Company,Shanghai, China). Briefly, VSMCs were seeded in 24-well plates at a density of 5 × 104 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, after which the cell culture supernatants were collected and centrifuged at 12 000 g for 15 min at 4 °C; the extracted supernatants were immediately frozen at ? 80 °C until use. Absorbance values at 450 nm were determined using an ELISA reader (Bio-Rad, CA, USA).

【文獻(xiàn)標(biāo)題】Effects of Selenium-Enriched Agaricus blazei Murill on Liver Metabolic Dysfunction in Mice, a Comparison with Selenium-Deficient Agaricus blazei Murill and Sodium Selenite

【作者】Lei Yu ,Shaolong Yang , Lei Sun,et.al

【作者單位】哈爾濱醫(yī)科大學(xué)（Harbin Medical University）

【文獻(xiàn)中引用產(chǎn)品】

小鼠白介素1β (IL-1β)ELISA試劑盒

小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒

【關(guān)鍵詞】Agaricus blazei Murill . ALT . AST . Alcoholichepatitis . Selenium

【DOI】doi.org/10.1007/s12011-014-0022-8

【出版期刊】《Biol Trace Elem Res》

【產(chǎn)品原文引用】

Estimation of Liver IL-1β and TNF-αLevel Liver samples were disintegrated in five volumes of ice-cold RIPA buffer. After incubation on ice for 30 min, samples were centrifuged twice at 20,000×g for 15 min at 4 °C. The resulting supernatants were used for assay. The hepatic concentration of interleukin-1β (IL-1β) and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.

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